Plasmid Coding for Drug Resistance and Production of Heat-Labile and Heat-Stable Toxins Harbored by an Escherichia coli Strain of Human Origin
نویسندگان
چکیده
منابع مشابه
Expression of proteins Heat-Labile (Lt-1) and Heat-Stable (STa) Escherichia coli expression of proteins Heat-Labile (Lt-1) and Heat-Stable (STa) Escherichia coli
Introduction Escherichia coli has a direct relationship with the diarrheagenic diseases, which occur quite in developing countries due to poor sanitation system and public health. It mainly affects children, causing a rate of morbidity and mortality worrying. This bacterium has a versatility of strains expressing different mechanisms and virulence factors, thus the importance in studying the ta...
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Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
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BACKGROUND Diarrhea is a prevalent pathological condition frequently associated to the colonization of the small intestine by enterotoxigenic Escherichia coli (ETEC) strains, known to be endemic in developing countries. These strains can produce two enterotoxins associated with the manifestation of clinical symptoms that can be used to detect these pathogens. Although several detection tests ha...
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With the plasmid DNA from a clinical isolate of enterotoxigenic Escherichia coli (ETEC) H 10407 as template, PCR-mediated cloning of the sequence encoding the heat-labile toxin B subunit (L T -B) has been carried out. Then this sequence was recloned into the pTrc 99A and pET23a expression vectors to give the pJasmids pTRCLTB and pETLTB, respectively. After induction, the former plasmid provides...
متن کاملDifferent Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates
Enterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1983
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.39.2.970-973.1983